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KMID : 0848120100350030091
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International Journal of Oral Biology
2010 Volume.35 No. 3 p.91 ~ p.97
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Effects of Chitosan on the Differentiation of MDPC-23 Cells
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Park Ju-Hyun
Kim Do-Kyung
Park Jong-Tae
Kim Su-Young
Yu Sun-Kyoung
Cho Kwang-Hee
Kim Heung-Joong
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Abstract
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The effects of chitosan upon the experimentally induced differentiation of MDPC-23 cells, derived from mouse dental papilla cells, were investigated by RT-PCR, observations of cell morphology and Alizaline red-S staining. Chitosan was found to significantly increase and accelerate the expression of ALP mRNA but decrease the ColI transcript levels, as compared with the control, in a time-dependent manner during the differentiation of MDPC-23 cells. Chitosan also significantly downregulated ON mRNA expression and accelerated mineralization in differentiating MDPC-23 cells. These results suggest that chitosan facilitates odontoblast differentiation and mineralization and may have potential clinical applications as a dentin regeneration material.
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KEYWORD
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chitosan, odontoblast, differentiation
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